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wild type wt k562 cells  (ATCC)
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ATCC wild type wt k562 cells
Characterization of transduced CAR/CXCR5 T cells produced from splenocytes of an hDRAGA mouse. (A) CAR/CXCR5 transduced cells from one DRAGA spleen were identified by expression of MBL and CXCR5. A gate was set on the control UTD cells (not shown). (B) CD4 + and CD8 + expression within MBL + CXCR5 + T cells. (C) Memory populations were gated within MBL + CXCR5 + transduced T cells and identified as naive or stem cell memory (SCM) (CD45RA + , CD45RO – ), central memory (CM) (CCR7 + , CD27 + ), transitional effector memory (TEM) (CCR7 – , CD27 + ), or effector memory (EM) (CCR7 – , CD27 + ). Gates were set on lymphocytes, singlets, and live human CD3 + T cells for (A), and additionally on MBL + CXCR5 + cells for (B) and (C). (D) Migration to CXCL13 by control UTD (gray) or CAR/CXCR5 T cells (blue) was measured in a transwell migration assay. (E) CAR/CXCR5 T cells were co-cultured with either WT (red) or HIV-env–expressing <t>K562</t> cells (blue) at a 1:3 E:T ratio and the expression of TNF-α, IFN-γ, and CD107a were measured. Gates were set on lymphocytes, singlets, and live human CD3 + T cells for (A), and on MBL + CXCR5 + cells for (D). (F) A DELFIA cytotoxicity assay was performed at 5:1, 10:1, and 20:1 E:T ratios with CAR/CXCR5 T cells plus HIV-env K562 cells (blue), CAR/CXCR5 T cells plus WT K562 cells (red), or UTD T cells plus HIV-env K562 cells (gray).
Wild Type Wt K562 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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wild type wt k562 cells - by Bioz Stars, 2026-09
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k562 wt cell line atcc ccl  (ATCC)
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ATCC k562 wt cell line atcc ccl
Characterization of transduced CAR/CXCR5 T cells produced from splenocytes of an hDRAGA mouse. (A) CAR/CXCR5 transduced cells from one DRAGA spleen were identified by expression of MBL and CXCR5. A gate was set on the control UTD cells (not shown). (B) CD4 + and CD8 + expression within MBL + CXCR5 + T cells. (C) Memory populations were gated within MBL + CXCR5 + transduced T cells and identified as naive or stem cell memory (SCM) (CD45RA + , CD45RO – ), central memory (CM) (CCR7 + , CD27 + ), transitional effector memory (TEM) (CCR7 – , CD27 + ), or effector memory (EM) (CCR7 – , CD27 + ). Gates were set on lymphocytes, singlets, and live human CD3 + T cells for (A), and additionally on MBL + CXCR5 + cells for (B) and (C). (D) Migration to CXCL13 by control UTD (gray) or CAR/CXCR5 T cells (blue) was measured in a transwell migration assay. (E) CAR/CXCR5 T cells were co-cultured with either WT (red) or HIV-env–expressing <t>K562</t> cells (blue) at a 1:3 E:T ratio and the expression of TNF-α, IFN-γ, and CD107a were measured. Gates were set on lymphocytes, singlets, and live human CD3 + T cells for (A), and on MBL + CXCR5 + cells for (D). (F) A DELFIA cytotoxicity assay was performed at 5:1, 10:1, and 20:1 E:T ratios with CAR/CXCR5 T cells plus HIV-env K562 cells (blue), CAR/CXCR5 T cells plus WT K562 cells (red), or UTD T cells plus HIV-env K562 cells (gray).
K562 Wt Cell Line Atcc Ccl, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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k562 wt cell line atcc ccl - by Bioz Stars, 2026-09
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wt k562 cell line  (Multiplexion GmbH)
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Multiplexion GmbH wt k562 cell line
Characterization of transduced CAR/CXCR5 T cells produced from splenocytes of an hDRAGA mouse. (A) CAR/CXCR5 transduced cells from one DRAGA spleen were identified by expression of MBL and CXCR5. A gate was set on the control UTD cells (not shown). (B) CD4 + and CD8 + expression within MBL + CXCR5 + T cells. (C) Memory populations were gated within MBL + CXCR5 + transduced T cells and identified as naive or stem cell memory (SCM) (CD45RA + , CD45RO – ), central memory (CM) (CCR7 + , CD27 + ), transitional effector memory (TEM) (CCR7 – , CD27 + ), or effector memory (EM) (CCR7 – , CD27 + ). Gates were set on lymphocytes, singlets, and live human CD3 + T cells for (A), and additionally on MBL + CXCR5 + cells for (B) and (C). (D) Migration to CXCL13 by control UTD (gray) or CAR/CXCR5 T cells (blue) was measured in a transwell migration assay. (E) CAR/CXCR5 T cells were co-cultured with either WT (red) or HIV-env–expressing <t>K562</t> cells (blue) at a 1:3 E:T ratio and the expression of TNF-α, IFN-γ, and CD107a were measured. Gates were set on lymphocytes, singlets, and live human CD3 + T cells for (A), and on MBL + CXCR5 + cells for (D). (F) A DELFIA cytotoxicity assay was performed at 5:1, 10:1, and 20:1 E:T ratios with CAR/CXCR5 T cells plus HIV-env K562 cells (blue), CAR/CXCR5 T cells plus WT K562 cells (red), or UTD T cells plus HIV-env K562 cells (gray).
Wt K562 Cell Line, supplied by Multiplexion GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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k562-hsp90β wt cells  (Corning Life Sciences)
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Corning Life Sciences k562-hsp90β wt cells
Characterization of transduced CAR/CXCR5 T cells produced from splenocytes of an hDRAGA mouse. (A) CAR/CXCR5 transduced cells from one DRAGA spleen were identified by expression of MBL and CXCR5. A gate was set on the control UTD cells (not shown). (B) CD4 + and CD8 + expression within MBL + CXCR5 + T cells. (C) Memory populations were gated within MBL + CXCR5 + transduced T cells and identified as naive or stem cell memory (SCM) (CD45RA + , CD45RO – ), central memory (CM) (CCR7 + , CD27 + ), transitional effector memory (TEM) (CCR7 – , CD27 + ), or effector memory (EM) (CCR7 – , CD27 + ). Gates were set on lymphocytes, singlets, and live human CD3 + T cells for (A), and additionally on MBL + CXCR5 + cells for (B) and (C). (D) Migration to CXCL13 by control UTD (gray) or CAR/CXCR5 T cells (blue) was measured in a transwell migration assay. (E) CAR/CXCR5 T cells were co-cultured with either WT (red) or HIV-env–expressing <t>K562</t> cells (blue) at a 1:3 E:T ratio and the expression of TNF-α, IFN-γ, and CD107a were measured. Gates were set on lymphocytes, singlets, and live human CD3 + T cells for (A), and on MBL + CXCR5 + cells for (D). (F) A DELFIA cytotoxicity assay was performed at 5:1, 10:1, and 20:1 E:T ratios with CAR/CXCR5 T cells plus HIV-env K562 cells (blue), CAR/CXCR5 T cells plus WT K562 cells (red), or UTD T cells plus HIV-env K562 cells (gray).
K562 Hsp90β Wt Cells, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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wt k562 cell line  (ATCC)
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ATCC wt k562 cell line
Characterization of transduced CAR/CXCR5 T cells produced from splenocytes of an hDRAGA mouse. (A) CAR/CXCR5 transduced cells from one DRAGA spleen were identified by expression of MBL and CXCR5. A gate was set on the control UTD cells (not shown). (B) CD4 + and CD8 + expression within MBL + CXCR5 + T cells. (C) Memory populations were gated within MBL + CXCR5 + transduced T cells and identified as naive or stem cell memory (SCM) (CD45RA + , CD45RO – ), central memory (CM) (CCR7 + , CD27 + ), transitional effector memory (TEM) (CCR7 – , CD27 + ), or effector memory (EM) (CCR7 – , CD27 + ). Gates were set on lymphocytes, singlets, and live human CD3 + T cells for (A), and additionally on MBL + CXCR5 + cells for (B) and (C). (D) Migration to CXCL13 by control UTD (gray) or CAR/CXCR5 T cells (blue) was measured in a transwell migration assay. (E) CAR/CXCR5 T cells were co-cultured with either WT (red) or HIV-env–expressing <t>K562</t> cells (blue) at a 1:3 E:T ratio and the expression of TNF-α, IFN-γ, and CD107a were measured. Gates were set on lymphocytes, singlets, and live human CD3 + T cells for (A), and on MBL + CXCR5 + cells for (D). (F) A DELFIA cytotoxicity assay was performed at 5:1, 10:1, and 20:1 E:T ratios with CAR/CXCR5 T cells plus HIV-env K562 cells (blue), CAR/CXCR5 T cells plus WT K562 cells (red), or UTD T cells plus HIV-env K562 cells (gray).
Wt K562 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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wt k562 cell line - by Bioz Stars, 2026-09
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nalm-6 and k562 isogenic cell lines with sf3b1 -k700k (wt) and sf3b1 -k700e (mt)  (H3 Biomedicine)
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H3 Biomedicine nalm-6 and k562 isogenic cell lines with sf3b1 -k700k (wt) and sf3b1 -k700e (mt)
Characterization of transduced CAR/CXCR5 T cells produced from splenocytes of an hDRAGA mouse. (A) CAR/CXCR5 transduced cells from one DRAGA spleen were identified by expression of MBL and CXCR5. A gate was set on the control UTD cells (not shown). (B) CD4 + and CD8 + expression within MBL + CXCR5 + T cells. (C) Memory populations were gated within MBL + CXCR5 + transduced T cells and identified as naive or stem cell memory (SCM) (CD45RA + , CD45RO – ), central memory (CM) (CCR7 + , CD27 + ), transitional effector memory (TEM) (CCR7 – , CD27 + ), or effector memory (EM) (CCR7 – , CD27 + ). Gates were set on lymphocytes, singlets, and live human CD3 + T cells for (A), and additionally on MBL + CXCR5 + cells for (B) and (C). (D) Migration to CXCL13 by control UTD (gray) or CAR/CXCR5 T cells (blue) was measured in a transwell migration assay. (E) CAR/CXCR5 T cells were co-cultured with either WT (red) or HIV-env–expressing <t>K562</t> cells (blue) at a 1:3 E:T ratio and the expression of TNF-α, IFN-γ, and CD107a were measured. Gates were set on lymphocytes, singlets, and live human CD3 + T cells for (A), and on MBL + CXCR5 + cells for (D). (F) A DELFIA cytotoxicity assay was performed at 5:1, 10:1, and 20:1 E:T ratios with CAR/CXCR5 T cells plus HIV-env K562 cells (blue), CAR/CXCR5 T cells plus WT K562 cells (red), or UTD T cells plus HIV-env K562 cells (gray).
Nalm 6 And K562 Isogenic Cell Lines With Sf3b1 K700k (Wt) And Sf3b1 K700e (Mt), supplied by H3 Biomedicine, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nalm-6 and k562 isogenic cell lines with sf3b1 -k700k (wt) and sf3b1 -k700e (mt) - by Bioz Stars, 2026-09
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nalm-6 and k562 isogenic cell lines with sf3b1-k700k (wt) and sf3b1-k700e (mt)  (H3 Biomedicine)
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H3 Biomedicine nalm-6 and k562 isogenic cell lines with sf3b1-k700k (wt) and sf3b1-k700e (mt)
Characterization of transduced CAR/CXCR5 T cells produced from splenocytes of an hDRAGA mouse. (A) CAR/CXCR5 transduced cells from one DRAGA spleen were identified by expression of MBL and CXCR5. A gate was set on the control UTD cells (not shown). (B) CD4 + and CD8 + expression within MBL + CXCR5 + T cells. (C) Memory populations were gated within MBL + CXCR5 + transduced T cells and identified as naive or stem cell memory (SCM) (CD45RA + , CD45RO – ), central memory (CM) (CCR7 + , CD27 + ), transitional effector memory (TEM) (CCR7 – , CD27 + ), or effector memory (EM) (CCR7 – , CD27 + ). Gates were set on lymphocytes, singlets, and live human CD3 + T cells for (A), and additionally on MBL + CXCR5 + cells for (B) and (C). (D) Migration to CXCL13 by control UTD (gray) or CAR/CXCR5 T cells (blue) was measured in a transwell migration assay. (E) CAR/CXCR5 T cells were co-cultured with either WT (red) or HIV-env–expressing <t>K562</t> cells (blue) at a 1:3 E:T ratio and the expression of TNF-α, IFN-γ, and CD107a were measured. Gates were set on lymphocytes, singlets, and live human CD3 + T cells for (A), and on MBL + CXCR5 + cells for (D). (F) A DELFIA cytotoxicity assay was performed at 5:1, 10:1, and 20:1 E:T ratios with CAR/CXCR5 T cells plus HIV-env K562 cells (blue), CAR/CXCR5 T cells plus WT K562 cells (red), or UTD T cells plus HIV-env K562 cells (gray).
Nalm 6 And K562 Isogenic Cell Lines With Sf3b1 K700k (Wt) And Sf3b1 K700e (Mt), supplied by H3 Biomedicine, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nalm-6 and k562 isogenic cell lines with sf3b1-k700k (wt) and sf3b1-k700e (mt) - by Bioz Stars, 2026-09
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wt and kdm3b ko k562 cells  (Shanghai Genechem Ltd)
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Shanghai Genechem Ltd wt and kdm3b ko k562 cells
Characterization of transduced CAR/CXCR5 T cells produced from splenocytes of an hDRAGA mouse. (A) CAR/CXCR5 transduced cells from one DRAGA spleen were identified by expression of MBL and CXCR5. A gate was set on the control UTD cells (not shown). (B) CD4 + and CD8 + expression within MBL + CXCR5 + T cells. (C) Memory populations were gated within MBL + CXCR5 + transduced T cells and identified as naive or stem cell memory (SCM) (CD45RA + , CD45RO – ), central memory (CM) (CCR7 + , CD27 + ), transitional effector memory (TEM) (CCR7 – , CD27 + ), or effector memory (EM) (CCR7 – , CD27 + ). Gates were set on lymphocytes, singlets, and live human CD3 + T cells for (A), and additionally on MBL + CXCR5 + cells for (B) and (C). (D) Migration to CXCL13 by control UTD (gray) or CAR/CXCR5 T cells (blue) was measured in a transwell migration assay. (E) CAR/CXCR5 T cells were co-cultured with either WT (red) or HIV-env–expressing <t>K562</t> cells (blue) at a 1:3 E:T ratio and the expression of TNF-α, IFN-γ, and CD107a were measured. Gates were set on lymphocytes, singlets, and live human CD3 + T cells for (A), and on MBL + CXCR5 + cells for (D). (F) A DELFIA cytotoxicity assay was performed at 5:1, 10:1, and 20:1 E:T ratios with CAR/CXCR5 T cells plus HIV-env K562 cells (blue), CAR/CXCR5 T cells plus WT K562 cells (red), or UTD T cells plus HIV-env K562 cells (gray).
Wt And Kdm3b Ko K562 Cells, supplied by Shanghai Genechem Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mrna sequencing wt k562 cells  (Shanghai Genechem Ltd)
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Shanghai Genechem Ltd mrna sequencing wt k562 cells
Characterization of transduced CAR/CXCR5 T cells produced from splenocytes of an hDRAGA mouse. (A) CAR/CXCR5 transduced cells from one DRAGA spleen were identified by expression of MBL and CXCR5. A gate was set on the control UTD cells (not shown). (B) CD4 + and CD8 + expression within MBL + CXCR5 + T cells. (C) Memory populations were gated within MBL + CXCR5 + transduced T cells and identified as naive or stem cell memory (SCM) (CD45RA + , CD45RO – ), central memory (CM) (CCR7 + , CD27 + ), transitional effector memory (TEM) (CCR7 – , CD27 + ), or effector memory (EM) (CCR7 – , CD27 + ). Gates were set on lymphocytes, singlets, and live human CD3 + T cells for (A), and additionally on MBL + CXCR5 + cells for (B) and (C). (D) Migration to CXCL13 by control UTD (gray) or CAR/CXCR5 T cells (blue) was measured in a transwell migration assay. (E) CAR/CXCR5 T cells were co-cultured with either WT (red) or HIV-env–expressing <t>K562</t> cells (blue) at a 1:3 E:T ratio and the expression of TNF-α, IFN-γ, and CD107a were measured. Gates were set on lymphocytes, singlets, and live human CD3 + T cells for (A), and on MBL + CXCR5 + cells for (D). (F) A DELFIA cytotoxicity assay was performed at 5:1, 10:1, and 20:1 E:T ratios with CAR/CXCR5 T cells plus HIV-env K562 cells (blue), CAR/CXCR5 T cells plus WT K562 cells (red), or UTD T cells plus HIV-env K562 cells (gray).
Mrna Sequencing Wt K562 Cells, supplied by Shanghai Genechem Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Characterization of transduced CAR/CXCR5 T cells produced from splenocytes of an hDRAGA mouse. (A) CAR/CXCR5 transduced cells from one DRAGA spleen were identified by expression of MBL and CXCR5. A gate was set on the control UTD cells (not shown). (B) CD4 + and CD8 + expression within MBL + CXCR5 + T cells. (C) Memory populations were gated within MBL + CXCR5 + transduced T cells and identified as naive or stem cell memory (SCM) (CD45RA + , CD45RO – ), central memory (CM) (CCR7 + , CD27 + ), transitional effector memory (TEM) (CCR7 – , CD27 + ), or effector memory (EM) (CCR7 – , CD27 + ). Gates were set on lymphocytes, singlets, and live human CD3 + T cells for (A), and additionally on MBL + CXCR5 + cells for (B) and (C). (D) Migration to CXCL13 by control UTD (gray) or CAR/CXCR5 T cells (blue) was measured in a transwell migration assay. (E) CAR/CXCR5 T cells were co-cultured with either WT (red) or HIV-env–expressing K562 cells (blue) at a 1:3 E:T ratio and the expression of TNF-α, IFN-γ, and CD107a were measured. Gates were set on lymphocytes, singlets, and live human CD3 + T cells for (A), and on MBL + CXCR5 + cells for (D). (F) A DELFIA cytotoxicity assay was performed at 5:1, 10:1, and 20:1 E:T ratios with CAR/CXCR5 T cells plus HIV-env K562 cells (blue), CAR/CXCR5 T cells plus WT K562 cells (red), or UTD T cells plus HIV-env K562 cells (gray).

Journal: The Journal of Immunology Author Choice

Article Title: Humanized DRAGA mice are a valuable model to study novel immunotherapies for HIV-1

doi: 10.1093/jimmun/vkaf185

Figure Lengend Snippet: Characterization of transduced CAR/CXCR5 T cells produced from splenocytes of an hDRAGA mouse. (A) CAR/CXCR5 transduced cells from one DRAGA spleen were identified by expression of MBL and CXCR5. A gate was set on the control UTD cells (not shown). (B) CD4 + and CD8 + expression within MBL + CXCR5 + T cells. (C) Memory populations were gated within MBL + CXCR5 + transduced T cells and identified as naive or stem cell memory (SCM) (CD45RA + , CD45RO – ), central memory (CM) (CCR7 + , CD27 + ), transitional effector memory (TEM) (CCR7 – , CD27 + ), or effector memory (EM) (CCR7 – , CD27 + ). Gates were set on lymphocytes, singlets, and live human CD3 + T cells for (A), and additionally on MBL + CXCR5 + cells for (B) and (C). (D) Migration to CXCL13 by control UTD (gray) or CAR/CXCR5 T cells (blue) was measured in a transwell migration assay. (E) CAR/CXCR5 T cells were co-cultured with either WT (red) or HIV-env–expressing K562 cells (blue) at a 1:3 E:T ratio and the expression of TNF-α, IFN-γ, and CD107a were measured. Gates were set on lymphocytes, singlets, and live human CD3 + T cells for (A), and on MBL + CXCR5 + cells for (D). (F) A DELFIA cytotoxicity assay was performed at 5:1, 10:1, and 20:1 E:T ratios with CAR/CXCR5 T cells plus HIV-env K562 cells (blue), CAR/CXCR5 T cells plus WT K562 cells (red), or UTD T cells plus HIV-env K562 cells (gray).

Article Snippet: Antigen-specific production of TNF-α and IFN-γ and surface expression of CD107a were assessed by co-culturing CAR/CXCR5 cells with HIV-env K562 cells (kindly provided by Dr James Riley, University of Pennsylvania) or wild-type (WT) K562 cells (ATCC) at an E:T ratio of 3:1.

Techniques: Produced, Expressing, Control, Migration, Transwell Migration Assay, Cell Culture, Cytotoxicity Assay